The CAMP Test - Scope, Principle, Test Procedure, Results and Interpretation
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Scope The presumptive identification of group B Streptococci can be done with the CAMP test. Principle The hemolytic phenomenon was first described in 1944 by Christie, Atkins, and Munch–Petersen, and it is their names that provide the acronym (CAMP) for the test. The hemolytic activity of the β-hemolysin produced by most strains of Staphylococcus aureus is enhanced by an extracellular protein produced by group B streptococci. Interaction of the β-hemolysin with this factor causes “synergistic hemolysis,” which is easily observed on a blood agar plate. This phenomenon is seen with both hemolytic and nonhemolytic isolates of group B streptococci. Other bacteria, such as Listeria monocytogenes, Rhodococcus equi, and certain strains of Vibrio cholera, are also CAMP-positive. Materials β-hemolysin-producing strain of Staphylococcus aureus Sheep Blood agar plate Quality Control Positive control: Group B Streptococcus Negative control: Group A Streptococcus Test ...