DETECTION OF EXTENDED-SPECTRUM β-LACTAMASES IN ENTEROBACTERIACEAE
Definition: Extended-spectrum β-Lactamase (ESBL) is an enzyme that hydrolyzes most penicillin and extended-spectrum cephalosporins with an oxyimino side chain. These cephalosporins include cefotaxime, ceftriaxone, and ceftazidime, as well as the oxyimino-monobactam (Aztreonam). ESBLs are inhibited by β-Lactamase inhibitors (Clavulanic acid, Sulbactam and Tazobactam). Aim: To identify and confirm the ability of the organism against Broad spectrum cephalosporins and monobactam. Principle: Resistant bacteria are emerging worldwide as a threat to the favorable outcome of common infections in community and hospital settings. β-lactamase production by several gram-negative organisms is perhaps the most important single mechanism of resistance to penicillins and cephalosporins. In the past it was believed that cephalosporins were relatively immune to attack by β-lactamases. It was surprising to find cephalosporin resistant Klebsiella spp. among the clinical iso...