Evaluation Of The Colistin Broth Disk Elution Method For Determine The MIC Of Colistin Against Gram Negative Bacilli
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Scope: To determine the minimum inhibitory concentration (MIC) in mcg/ml of colistin against microorganisms . Principle: The disk elution method is a quantitative technique performed in vitro, for determining the MIC of colistin against microorganisms. Material Required: a) Cation Adjusted Muller Hinton Broth b) Colistin sulfate disk (10µl) c) Saline d) Screw cap Tubes Equipment Required: a) Biological Safety Cabinet. b) Incubator Procedure: Standardized Inoculum Preparation Using a loop pick 3–5 colonies from a fresh (18–24 hours) nonselective agar plate and transfer to sterile saline (4–5 mL). Adjust turbidity to equivalent of a 0.5 McFarland turbidity standard . Test procedure: 1. Let the CAMHB tubes (10 mL) and colistin disks warm to room temperature. Label 4 tubes of CAMHB for each isolate to be tested with 1, 2, and 4 µg/mL and control. 2. Using asept...