Novobiocin-Susceptibility Test- Principle, Procedure and Results
.bmp)
Purpose This test is used to determine the effect of novobiocin on an organism. Staphylococcus saprophyticus is inhibited by novobiocin; whereas S. epidermis is not. Principle Novobiocin is an aminocoumarin antibiotic, produced by the actinomycete Streptomyces nivens, with antibacterial property. In 1975, Kloos and Schleifer reported a simplified scheme for differentiating coagulase-negative Staphylococcus spp. which included a novobiocin disk test. Coagulase-negative staphylococci can be divided into novobiocin-susceptible and novobiocin-resistant species. Among the novobiocin-resistant species, S. saprophyticus is the one commonly recovered from humans as a cause of urinary tract infections. Therefore, screening coagulase-negative staphylococci isolated from quantitative urine cultures for susceptibility to novobiocin provides a reliable presumptive identification of this species. Reagents Novobiocin disks, 5 mg Blood agar /Mueller Hinton agar plate ...