Flagella Stain- Principle, Procedure and Results Interpretation
.bmp)
Objective of Test This test used to presumptive identification of motile bacterial species from non motile bacteria by presence and arrangement of flagella. There are four types of flagellar bacteria based on presence and arrangement of flagella, such as Monotrichous, Amphitrichous, Lophotrichous and Peritrichous. Principle Most motile bacteria possess flagella, the shape, number, and position of which are important characteristics in the differentiation of genera and species identification, particularly when biochemical reactions are weak or equivocal. The Leifson staining technique (or modification) is most commonly used in clinical laboratories and is not difficult to perform, providing exact details that are followed in each step of the procedure. Bacterial flagella can be stained by alcoholic solutions of rosaniline dyes that form a precipitate as the alcohol evaporates in the staining procedure. Basic fuchsin (pararosaniline acetate) serves as the primary stain wi...