Furazolidone Disk Test- Principle, Procedure and Results
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Purpose Furazolidone susceptibility testing is a reliable method for differentiation of Staphylococci from Micrococci based on their susceptible. Principle There are several methods available for differentiation of staphylococci from micrococci. One of these is susceptibility to the compound furoxone (also called furazolidone). This test is performed as a disk susceptibility procedure; Staphylococci are susceptible to this compound, whereas micrococci are resistant. Media and Reagents Furazolidone disks, 100 mg Mueller Hinton Agar Quality Control Known strains of Staphylococcus (either S. aureus or a coagulase-negative strain) and Micrococcus species should be tested with each new lot of disks, or on a weekly basis. Test Procedure Prepare a suspension of the organism to be tested in sterile peptone water. The suspension should be equivalent to a 0.5 McFarland turbidity standard . Spread the organism suspension onto MHA plate by using swab. Aseptically plac...